Rice Straw Compost and Husk Biochar as Growth Starters and In Vitro Heavy Metal Biosorbents

Nurul Puspita Palupi, Esti Handayani Hardi, Fahrunsyah, Dwi Ermawati Rahayu, Rudy Agung Nugroho, Surya Darma, Suria Darma Idris, Arya Baya Sripati


Soil microbial activity is known to increase with compost application, aiding in nutrient absorption by plants and the provision of growth-promoting chemicals. Biocar, derived from plant fires or through heavy metal biosorption, also plays a crucial role in soil improvement. Therefore, this study aimed to develop rice straw compost and husk biochar as growth starters and evaluate their potential for heavy metal biosorption in vitro. The experiment was conducted in Samarinda, East Kalimantan, Indonesia, from October 2023 to January 2024. Rice straw and husk were collected at several random points in previously determined active fields (Stage I Point 4). After characterizing the chemical and physical properties, the rice straw was composted, and the husk was biocharized. The incubation medium used was rice field soil from Samarinda, while straw compost, husk biochar, soil compounds, and heavy metals were evaluated at the Soil Science Laboratory, Mulawarman University. In addition, microbial analyses were performed at the Plant Pest and Disease Laboratory, Faculty of Agriculture, and Microbiology Laboratory, Faculty of Fisheries and Marine Sciences. The results showed that compost materials and biochar content strongly influenced the overall bacterial and fungal colonies. As the incubation time increased, the bacterial and fungal colonies decreased. During the initial two months of waste intake, the number of colonies significantly increased, showing a notable difference compared to periods before the addition of compost or biochar.


Keywords: rice straw compost; husk biochar; biosorbent; bacteria; fungi




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SABIR A, ALTAF F, BATOOL R, et al. Agricultural waste absorbents for heavy metal removal. In: INAMUDDIN, AHAMED M, LICHTFOUSE E, et al. (eds.) Green adsorbents to remove metals, dyes and boron from polluted water. Environmental chemistry for a sustainable world. Cham: Springer, 2021, Volume 49: 195–228.

ADEGBEYE M J, SALEM A Z M, REDDY P R K, et al. Waste recycling for the eco-friendly input use efficiency in agriculture and livestock feeding. In: KUMAR S, MEENA R S, JHARIYA M K. (eds.) Resources use efficiency in agriculture. Singapore: Springer, 2020: 1–45.

CICCULLO F, CAGLIANO R, BARTEZZAGHI G, et al. Implementing the circular economy paradigm in the agri-food supply chain: the role of food waste prevention technologies. Resources, Conservation and Recycling, 2021, 164, 105114.

KOUL B, YAKOOB M, SHAH M P. Agricultural waste management strategies for environmental sustainability. Environmental Research, 2022, 206, 112285.

MARBUN T D, LEE K, SONG J, et al. Effect of lactic acid bacteria on the nutritive value and in vitro ruminal digestibility of maize and rice straw silage. Applied Sciences, 2020, 10(21), 7801.

MERAL R, KOSE Y E, CEYLAN Z, et al. Chapter 10 - The potential use of agro-industrial by-products as sources of bioactive compounds: a nanotechnological approach. Studies in Natural Products Chemistry, 2022, 73, 435–466.

DEVIANTI, YUSMANIZAR Y, SYAKUR S, et al. Organic fertilizer from agricultural waste: determination of phosphorus content using near infrared reflectance. IOP Conference Series: Earth and Environmental Science, 2020, 644, 012002.

INNOCENTI F D. Is composting of packaging real recycling? Waste Management, 2021, 130, 61–64.

GREFF B, SZIGETI J, NAGY A, et al. Influence of microbial inoculants on co-composting of lignocellulosic crop residues with farm animal manure: a review. Journal of Environmental Management, 2022, 302(Part B), 114088.

BHATTACHARYYA P, BHADURI D, ADAK T, et al. Characterization of rice straw from major cultivars for best alternative industrial uses to cutoff the menace of straw burning. Industrial Crops and Products, 2020, 143, 111919.

SINGH T B, ALI A, PRASAD M, et al. Role of organic fertilizers in improving soil fertility. In: NAEEM M, ANSARI A, GILL S. (eds.) Contaminants in agriculture. Cham: Springer, 2020: 61–77.

LI C, LI H, YAO T, et al. Effects of swine manure composting by microbial inoculation: heavy metal fractions, humic substances, and bacterial community metabolism. Journal of Hazardous Materials, 2021, 415, 125559.

AYILARA M S, OLANREWAJU O S, BABALOLA O O, et al. Waste management through composting: challenges and potentials. Sustainability, 2020, 12(11), 4456.

ABDELLAH Y A Y, ZANG H, LI C. Steroidal estrogens during composting of animal manure: persistence, degradation, and fate, a review. Water, Air, & Soil Pollution, 2020, 231, 547.

SCHMIDT H P, KAMMANN C, HAGEMANN N, et al. Biochar in agriculture – a systematic review of 26 global meta-analyses. GCB Bioenergy: Bioproducts for a Sustainable Bioeconomy, 2021, 13(11), 1708–1730.

SONG B, ALMATRAFI E, TAN X, et al. Biochar-based agricultural soil management: an application-dependent strategy for contributing to carbon neutrality. Renewable and Sustainable Energy Reviews, 2022, 164, 112529.

MEENA H M, PRAKASHA H C. Effect of biochar, lime and soil test value based fertilizer application on soil fertility, nutrient uptake and yield of rice-cowpea cropping system in an acid soil of Karnataka. Journal of Plant Nutrition, 2020, 43, 2664–2679.

VELMURUGAN V. Review of research and development on pyrolysis process. Materials Today: Proceedings, 2022, 49, 3679–3686.

MINISTRY OF STATE FOR POPULATION AND ENVIRONMENT, REPUBLIC OF INDONESIA, DALHOUSIE UNIVERSITY, CANADA. Environmental management in Indonesia. Report on soil quality standards for Indonesia (interim report). 1992.

CONG P, WANG J, LI Y, et al. Changes in soil organic carbon and microbial community under varying straw incorporation strategies. Soil and Tillage Research, 2020, 204, 104735.

LI Z, SHEN Y, ZHANG W, et al. Effects of long-term straw returning on rice yield and soil properties and bacterial community in a rice-wheat rotation system. Field Crops Research, 2023, 291, 108800.

BEI S, LI X, KUYPER T W, et al. Nitrogen availability mediates the priming effect of soil organic matter by preferentially altering the straw carbon-assimilating microbial community. Science of the Total Environment, 2022, 815, 152882.

SIEDT M, SCHÄFFER A, SMITH K E, et al. Comparing straw, compost, and biochar regarding their suitability as agricultural soil amendments to affect soil structure, nutrient leaching, microbial communities, and the fate of pesticides. Science of the Total Environment, 2021, 751, 141607.

FAN S, ZUO J, DONG H. Changes in soil properties and bacterial community composition with biochar amendment after six years. Agronomy, 2020, 10(5), 746.

GAO S, DELUCA T H. Biochar alters nitrogen and phosphorus dynamics in a western rangeland ecosystem. Soil Biology and Biochemistry, 2020, 148, 107868.

HOU J, PUGAZHENDHI A, PHUONG T N, et al. Plant disease resistance: using biochar to inhibit harmful microbes and absorb nutrients. Environmental Research, 2022, 214(Part 2), 113883.

GUAN K X, WEI L, TURNER N C, et al. Improved straw management practices promote in situ straw decomposition and nutrient release and increase crop production. Journal of Cleaner Production, 2020, 250, 119514.

WU L, ZHANG W, WEI W, et al. Soil organic matter priming and carbon balance after straw addition is regulated by long-term fertilization. Soil Biology and Biochemistry, 2019, 135, 383–391.

MILKEREIT J, GEISSELER D, LAZICKI P, et al. Interactions between nitrogen availability, bacterial communities, and nematode indicators of soil food web function in response to organic amendments. Applied Soil Ecology, 2021, 157, 103767.

SIEDT M, SCHÄFFER A, SMITH K E C, et al. Comparing straw, compost, and biochar regarding their suitability as agricultural soil amendments to affect soil structure, nutrient leaching, microbial communities, and the fate of pesticides. Science of the Total Environment, 2021, 751, 141607.

VAHEDI R, SADAGHIANI M R, BARIN M, et al. Interactions between biochar and compost treatment and mycorrhizal fungi to improve the qualitative properties of a calcareous soil under rhizobox conditions. Agriculture, 2021, 11(10), 993.

LAOUANE R B, BASLAM M, MOKHTAR M A L, et al. Potential of native arbuscular mycorrhizal fungi, rhizobia, and/or green compost as alfalfa (Medicago sativa) enhancers under salinity. Microorganisms, 2020, 8(11), 1695.

MORENO J L, BASTIDA F, LÓPEZM D, et al. Response of soil chemical properties, enzyme activities and microbial communities to biochar application and climate change in a Mediterranean agroecosystem. Geoderma, 2022, 407, 115536.

PANAHI H K S, DEHHAGHI M, OK Y S, et al. A comprehensive review of engineered biochar: production, characteristics, and environmental applications. Journal of Cleaner Production, 2020, 270, 122462.

WANG D, ZHOU Y, ZHAO P, et al. Maize-potato residue mixing in agricultural soils enhances residue decomposition and stable carbon content by modifying the potential keystone microbial taxa. Geoderma, 2023, 437, 116581.

PHOUM R, SEHRAWAT A, SINDHU S S, et al. Interkingdom signaling in plant-rhizomicrobiome interactions for sustainable agriculture. Microbiological Research, 2020, 241, 126589.

ROMBEL A, KRASUCKA P, OLESZCZUK P. Sustainable biochar-based soil fertilizers and amendments as a new trend in biochar research. Science of the Total Environment, 2022, 816, 151588.

YUAN M, ZHU X, SUN H, et al. The addition of biochar and nitrogen alters the microbial community and their cooccurrence network by affecting soil properties. Chemosphere, 2023, 312(Part 1), 137101.

WEN Z, CHEN Y, LIU Z, et al. Biochar and arbuscular mycorrhizal fungi stimulate rice root growth strategy and soil nutrient availability. European Journal of Soil Biology, 2023, 113, 103448.

CHEN Y, WEN Z, MENG J, et al. The positive effects of biochar application on Rhizophagus irregularis, rice seedlings, and phosphorus cycling in paddy soil. Pedosphere, 2024, 34(2), 361–373.

GUJRE N, SONI A, RANGAN L, et al. Sustainable improvement of soil health utilizing biochar and arbuscular mycorrhizal fungi: a review. Environmental Pollution, 2023, 268(Part B), 115549.

HALIM M A, RAHMAN M M, MEGHARAJ M, et al. Cadmium immobilization in the rhizosphere and plant cellular detoxification: role of plant-growth-promoting rhizobacteria as a sustainable solution. Journal of Agricultural and Food Chemistry, 2020, 68(47), 13497–13529.

NIVETHA N, SRIVARSHINE B, SOWMYA B, et al. A comprehensive review on bio-stimulation and bio-enhancement towards remediation of heavy metals degeneration. Chemosphere, 2023, 312(Part 1), 137099.

KAZEMALILOU S, DELANGIZ N, LAJAYERB A, et al. Chapter 9 - Insight into plant-bacteria-fungi interactions to improve plant performance via remediation of heavy metals: an overview. In: SHARMA V, SALWAN R, AL-ANI L K T. (eds.) Molecular aspects of plant beneficial microbes in agriculture. Elsevier, 2020: 123–132. https://doi.org/10.1016/B978-0-12-818469-1.00010-9

TEDERSO L, BAHRAM M, ZOBEL M. How mycorrhizal associations drive plant population and community biology. Science, 2020, 367(6480), eaba1223.

LIU S, YANG B, LIANG Y, et al. Prospect of phytoremediation combined with other approaches for remediation of heavy metal-polluted soils. Environmental Science and Pollution Research, 2020, 27, 16069–16085.

PERIS V V, OLLAS C D, CADENAS A G, et al. Root exudates: from plant to rhizosphere and beyond. Plant Cell Reports, 2020, 39(1), 3–17.

DHALARIA R, KUMAR D, KUMAR H, et al. Arbuscular mycorrhizal fungi as potential agents in ameliorating heavy metal stress in plants. Agronomy, 2020, 10(6), 815.


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