Potential of Bacterial Isolates and Consortiums for Degrading Linear Alkylbenzenesulfonate, Ammonia, and Phosphate in the Kaliotic River
Abstract
River pollution by various organic and inorganic compounds such as linear alkylbenzenesulfonate (LAS), ammonia, and phosphate is a serious environmental problem in the Kaliotic River. This research investigates the potential of bacterial isolates and consortia for degrading these pollutants. We conducted a field survey and collected water samples from the Kaliotic River and isolated bacteria from these water samples. Next, we tested the ability of single isolates and bacterial consortia to degrade LAS, ammonia, and phosphate in vitro. The results showed that various bacterial isolates isolated from the Kaliotic River showed varying abilities to degrade LAS, ammonia, and phosphate. Some single isolates could degrade LAS to a significant extent, whereas other isolates showed higher activity in reducing ammonia and phosphate concentrations in the medium. The bacterial consortium consisting of several isolates also showed promising potential for degrading these three pollutants. Further analysis using molecular techniques such as 16S rRNA DNA sequencing shows the genetic diversity of bacteria involved in pollutant degradation. This study also highlights the importance of environmental factors such as temperature, pH, and nutrient availability in influencing bacterial degradation activity. These findings indicate that bacterial isolates and consortia have great potential for degrading LAS, ammonia, and phosphate in the Kaliotic River. This research provides new insights into the potential application of microbes in environmental remediation efforts in polluted rivers. By better understanding the mechanisms of degradation and the factors that influence bacterial activity, it is hoped that more effective and sustainable strategies can be developed to rid rivers of these dangerous pollutants.
Keywords: bacterial isolates; consortiums; the Kaliotic River
DOI：10.62321/issn.1000-1298.2024.05.06
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TUDI M, DANIEL RUAN H, WANG L, et al. Agriculture development, pesticide application and its impact on the environment. International Journal of Environmental Research and Public Health, 2021, 18(3), 1112.
GEBREMICHAEL S G, YISMAW E, TSEGAW B D, et al. Determinants of water source use, quality of water, sanitation and hygiene perceptions among urban households in North-West Ethiopia: a cross-sectional study. PLoS ONE, 2021, 16(4), e0239502.
SINGH N, POONIA T, SIWAL S S, et al. Challenges of water contamination in urban areas. Current Directions in Water Scarcity Research, 2022, 6, 173–202.
KAUR T, SINHA A K. Pesticides in agricultural run offs affecting water resources: a study of Punjab (India). Agricultural Sciences, 2019, 10(10), 1381–1395.
JAIN C K, SINGH S. Impact of climate change on the hydrological dynamics of River Ganga, India. Journal of Water and Climate Change, 2020, 11(1), 274–290.
MADHAV S, AHAMAD A, SINGH A K, et al. Water pollutants: sources and impact on the environment and human health. In: POOJA D, KUMAR P, SINGH P, et al. (eds.) Sensors in water pollutants monitoring: role of material. Advanced functional materials and sensors. Singapore: Springer, 2020: 43–62.
LATA S. Sustainable and eco-friendly approach for controlling industrial wastewater quality imparting succour in water-energy nexus system. Energy Nexus, 2021, 3, 100020.
LUO Y, JIN X, XIE H, et al. Linear alkylbenzene sulfonate threats to surface waters at the national scale: a neglected traditional pollutant. Journal of Environmental Management, 2023, 342, 118344.
AKHTAR N, SYAKIR ISHAK M I, BHAWANI S A, et al. Various natural and anthropogenic factors responsible for water quality degradation: a review. Water, 2021, 13(19), 2660.
TIWARI A K, PAL D B. Nutrients contamination and eutrophication in the river ecosystem. In: MADHAV S, KANHAIYA S, SRIVASTAV A, et al. (eds.) Ecological significance of river ecosystems: challenges and management strategies. Elsevier, 2022: 203–216. https://doi.org/10.1016/B978-0-323-85045-2.00001-7
CAO Z, YAN W, DING M, et al. Construction of microbial consortia for microbial degradation of complex compounds. Frontiers in Bioengineering and Biotechnology, 2022, 10, 1051233.
GULSUNOGLU-KONUSKAN Z, KILIC-AKYILMAZ M. Microbial bioconversion of phenolic compounds in agro-industrial wastes: a review of mechanisms and effective factors. Journal of Agricultural and Food Chemistry, 2022, 70(23), 6901–6910.
MALANOTTE-RIZZOLI P, ARTALE V, BORZELLI-EUSEBI G L, et al. Physical forcing and physical/biochemical variability of the Mediterranean Sea: a review of unresolved issues and directions for future research. Ocean Science, 2014, 10(3), 281–322.
ZHANG B, LI W, GUO Y, et al. Microalgal-bacterial consortia: from interspecies interactions to biotechnological applications. Renewable and Sustainable Energy Reviews, 2020, 118, 109563.
WONDRACZEK L, POHNERT G, SCHACHER F H, et al. Artificial microbial arenas: materials for observing and manipulating microbial consortia. Advanced Materials, 2019, 31(24), 1900284.
SPINI G, SPINA F, POLI A, et al. Molecular and microbiological insights on the enrichment procedures for the isolation of petroleum degrading bacteria and fungi. Frontiers in Microbiology, 2018, 9, 2543.
LATIF A, SHENG D, SUN K, et al. Remediation of heavy metals polluted environment using Fe-based nanoparticles: mechanisms, influencing factors, and environmental implications. Environmental Pollution, 2020, 264, 114728.
TOMAK A, CESMELI S, HANOGLU B D, et al. Nanoparticle-protein corona complex: understanding multiple interactions between environmental factors, corona formation, and biological activity. Nanotoxicology, 2021, 15(10), 1331–1357.
VEACH A M, TROIA M J, CREGGER M A. Assessing biogeographic survey gaps in bacterial diversity knowledge: a global synthesis of freshwaters. Freshwater Biology, 2021, 66(8), 1595–1605.
PAL A, HE Y, JEKEL M, et al. Emerging contaminants of public health significance as water quality indicator compounds in the urban water cycle. Environment International, 2014, 71, 46–62.
RAPTIS C E, VAN VLIET M T, PFISTER S. Global thermal pollution of rivers from thermoelectric power plants. Environmental Research Letters, 2016, 11(10), 104011.
HANAFI M F, SAPAWE N. A review on the water problem associate with organic pollutants derived from phenol, methyl orange, and remazol brilliant blue dyes. Materials Today: Proceedings, 2020, 31, A141–A150.
AYILARA M S, OLANREWAJU O S, BABALOLA O O, et al. Waste management through composting: challenges and potentials. Sustainability, 2020, 12(11), 4456.
DA COSTA J P, MOUNEYRAC C, COSTA M, et al. The role of legislation, regulatory initiatives and guidelines on the control of plastic pollution. Frontiers in Environmental Science, 2020, 8, 104.
FUENTES B, CHOQUE A, CASTRO-NALLAR E, et al. Influence of physical-chemical soil parameters on microbiota composition and diversity in a deep hyperarid core of the Atacama Desert. Frontiers in Microbiology, 2022, 12, 794743.
LIU J, FENG Y, ZHANG Y, et al. Allometric releases of nitrogen and phosphorus from sediments mediated by bacteria determines water eutrophication in coastal river basins of Bohai Bay. Ecotoxicology and Environmental Safety, 2022, 235, 113426.
WORLD HEALTH ORGANIZATION. Guidelines for drinking-water quality: fourth edition incorporating the first and second addenda, 2022. https://www.pseau.org/outils/ouvrages/who_guidelines_for_drinking_water_quality_4th_edition_2022.pdf
BALA S, GARG D, THIRUMALESH B V, et al. Recent strategies for bioremediation of emerging pollutants: a review for a green and sustainable environment. Toxics, 2022, 10(8), 484.
AYILARA M S, BABALOLA O O. Bioremediation of environmental wastes: the role of microorganisms. Frontiers in Agronomy, 2023, 5, 1183691.
DAS S, KUPPANAN N, CHANNASHETTAR V A, et al. Remediation of oily sludge- and oil-contaminated soil from petroleum industry: recent developments and future prospects. In: ADHYA T, LAL B, MOHAPATRA B, et al. (eds.) Advances in soil microbiology: recent trends and future prospects. Microorganisms for sustainability. Singapore: Springer, 2018, Volume 3: 165–177.
THACHARODI A, HASSAN S, SINGH T, et al. Bioremediation of polycyclic aromatic hydrocarbons: an updated microbiological review. Chemosphere, 2023, 328, 138498.
LIU C J, DENG S G, HU C Y, et al. Applications of bioremediation and phytoremediation in contaminated soils and waters: CREST publications during 2018–2022. Critical Reviews in Environmental Science and Technology, 2023, 53(6), 723–732.
BERHANU A, MUTANDA I, TAOLIN J, et al. A review of microbial degradation of per- and polyfluoroalkyl substances (PFAS): biotransformation routes and enzymes. Science of the Total Environment, 2023, 859, 160010.
AHMED S F, MOFIJUR M, PARISA T A, et al. Progress and challenges of contaminate removal from wastewater using microalgae biomass. Chemosphere, 2022, 286, 131656.
DUAN L, LI J L, YIN L Z, et al. Habitat-dependent prokaryotic microbial community, potential keystone species, and network complexity in a subtropical estuary. Environmental Research, 2022, 212, 113376.
RAFEEQ H, AFSHEEN N, RAFIQUE S, et al. Genetically engineered microorganisms for environmental remediation. Chemosphere, 2023, 310, 136751.
OLIVEIRA V H, DÍEZ S, DOLBETH M, et al. Restoration of degraded estuarine and marine ecosystems: a systematic review of rehabilitation methods in Europe. Journal of Hazardous Materials, 2024, 469, 133863.
SARAVANAN A, KUMAR P S, DUC P A, et al. Strategies for microbial bioremediation of environmental pollutants from industrial wastewater: a sustainable approach. Chemosphere, 2023, 313, 137323.
SIGALLA O Z, TUMBO M, JOSEPH J. Multi-stakeholder platform in water resources management: a critical analysis of stakeholders’ participation for sustainable water resources. Sustainability, 2021, 13(16), 9260.
SONG Y, HOU D, ZHANG J, et al. Environmental and socio-economic sustainability appraisal of contaminated land remediation strategies: a case study at a mega-site in China. Science of the Total Environment, 2018, 610, 391–401.
WU L, YANG Y, GUO W, et al. Deterioration of biological pollutants removal induced by linear alkylbenzene sulphonates in sequencing batch reactors: insight of sludge characteristics, microbial community and metabolic activity. Bioresource Technology, 2020, 315, 123843.
TERRONES-FERNANDEZ I, RODERO-DE-LAMO L, LÓPEZ A, et al. Microwave oven application for the preparation and sterilization of microbiological culture media: a feasible method with an adapted water bath and perforable cap. Applied Sciences, 2024, 14(6), 2340.
RANTAŠA M, MAJER D, FINŠGAR M. Author spotlight: technologies and challenges in elemental analysis of food samples. Journal of Visualized Experiments, 2023, 202, e65624.
SELIM S A, ADAM M E, HASSAN S M, et al. Chemical composition, antimicrobial and antibiofilm activity of the essential oil and methanol extract of the Mediterranean cypress (Cupressus sempervirens L.). BMC Complementary and Alternative Medicine, 2014, 14, 179.
ALEMNEH A A, ZHOU Y, RYDER M H, et al. Is phosphate solubilizing ability in plant growth‐promoting rhizobacteria isolated from chickpea linked to their ability to produce ACC deaminase? Journal of Applied Microbiology, 2021, 131(5), 2416–2432.
ZHANG L, HU J, ZHU R, et al. Degradation of paracetamol by pure bacterial cultures and their microbial consortium. Applied Microbiology and Biotechnology, 2013, 97, 3687–3698.
ZABED H M, AKTER S, TIAN Y, et al. Assessing microbial systems and process configurations for improved ethanol production from sugary stovers by integrating soluble sugars and holocellulose. Industrial Crops and Products, 2024, 212, 118269.
CHEW K W, CHIA S R, SHOW P L, et al. Effects of water culture medium, cultivation systems and growth modes for microalgae cultivation: a review. Journal of the Taiwan Institute of Chemical Engineers, 2018, 91, 332–344.
ELTARAHONY M, EL-FAKHARANY E, ABU-SERIE M, et al. Statistical modeling of methylene blue degradation by yeast-bacteria consortium; optimization via agro-industrial waste, immobilization and application in real effluents. Microbial Cell Factories, 2021, 20, 234.
QIAN X, CHEN L, SUI Y, et al. Biotechnological potential and applications of microbial consortia. Biotechnology Advances, 2020, 40, 107500.
MONDAL S, HALDER S K, YADAV A N, et al. Microbial consortium with multifunctional plant growth-promoting attributes: future perspective in agriculture. In: YADAV A, RASTEGARI A, YADAV N, et al. (eds.) Advances in plant microbiome and sustainable agriculture. Microorganisms for sustainability. Singapore: Springer, 2020, Volume 20: 219–258.
AHMAD T, GUPTA G, SHARMA A, et al. Metagenomic analysis exploring taxonomic and functional diversity of bacterial communities of a Himalayan urban fresh water lake. PLoS ONE, 2021, 16(3), e0248116.
EL HAMSS H, RADOUANE N, BELABESS Z, et al. Microbial consortia: an approach to enhance the effectiveness of beneficial soil microbes. In: KHAN M R. (ed.) Novel biological and biotechnological applications in plant nematode management. Singapore: Springer, 2023: 133–166.
MALLA M A, DUBEY A, YADAV S, et al. Understanding and designing the strategies for the microbe-mediated remediation of environmental contaminants using omics approaches. Frontiers in Microbiology, 2018, 9, 1132.
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